Antibacterial activity of brown algae extract in Sargassum filipendulla from southeast maluku waters toward the Vibrio harveyii and Pseudomonas fluorescene bacteria

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Nally YGF Erbabley, Junianto, Edy Afrianto and Iskandar

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Abstract. Vibrio harveyii and Pseudomonas fluorescence bacteria are pathogenic bacteria that are often a problem in aquaculture, so effective antibacterial compounds are needed to inhibit their growth. Brown algae of Sargassumfilipendulla have bioactive compounds that can function as antibacterial compounds. The purpose of this study was to determine the phytochemical content of S. filipendulla of algae extract from Southeast Maluku waters and to determine the antibacterial activity of S. filipendulla extract to V. harveyii and P.fluorescence bacteria. Phytochemical test parameters were carried out quantitatively; antibacterial activity was carried out using agar diffusion method (Kirby Bauer). Data from the inhibitory zone test results were analyzed using the ANOVA (Analysis of variance) test followed by the Least Significant Difference (LSD) test. The test results showed that the brown algae of S. filipendulla originating from the Southeast Maluku region, produced extracts in the form of green paste to blackish green with the highest rendemen value in ethanol extract. The results of phytochemical screening showed that the compounds contained in S.filipendulla extract were phenolic, tannin, flavonoid, saponin, triterpenoid, steroid and alkaloid compounds; with the dominant compounds in the three reactants were saponin compounds .The results of the research of inhibition zones showed that the concentration of 100% had the highest inhibition zone value and the lowest inhibition zone at a concentration of 75%. Ethanol extract has a high value to inhibit pathogenic bacterium V.harveyi and P. fluorescence and the lowest on ethyl acetate extract, whereas positive control is suspect against bacteria and negative control does not affect pathogenic bacteria. The 100% concentration of ethanol extract gave a significant effect on inhibiting pathogenic bacteria V.harveyi and P. fluorescence (P <0.05). The 50% concentration gives a significant difference between ethanol and ethyl acetate extract, at the level of 0.05 (P <0.05), while the n-hexane extract gives a non-significant result of ethanol and ethyl acetate extract. The average extract value to inhibit the highest pathogenic bacteria is in ethanol extract and the lowest is in n-hexane extract. Of the three extracts used, ethanol extract is an extract that is good for inhibiting pathogenic bacteria and has bacteriostatic properties.

Key Words: Antibacterial Activity, Sargassum filipendulla algae, Southeast Maluku, Vibrio harveyi and Pseudomonuas fluorescence.

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