Abstract. Sea cucumber, Holothuria scabra is elongated round with mouth and anus lying on both sides of the opposite body. High protein content and contain anti-bacterial compounds because sandfish are widely caught and used as food and medicine. To maintain the availability of sandfish in a sustainable manner, it is necessary to do the breeding activities. This research aims to determine the spawning time and body weight of sandfish, H. scabra which can be used as the adult. The research took place from March to April 2018 where the adult sample was collected from the coastal of the Labetawi village and breaded in the Hatchery laboratory. Spawning stimulation was done by temperature fluctuation method. Results show that Sea Cucumber, Holothuria scabra, in the village of Labetawi matured and spawned in March and April. In addition, it is also known that sandfish, Holothuria scabra matured and spawned when it had a weight of 80 gram, where it can be identified as adult.